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3 plasmids (pGL1124, pGL1224 and pGL1217) have been created to allow the alternative of 1 allele of CYC9Lister 427 cells. Trypanosomes ended up transfected with Every plasmid independently, and after that subjected to the second transfection with one of many other plasmids, with another resistance gene, with all pairwise combinations done.To achiev